The study of the microanatomy of cells and their formation into tissues and organs as seen through a microscope. Histology forms the basis of much of our understanding of cell function and disease processes. Our facilities provide equipment for sectioning fresh or fixed tissues in thin slices, as well as resources for visualization of proteins or other components of interest within the tissue.
Sectioning of tissue
The facilities offer two Thermo Scientific HM 430 freezing microtomes with external freezing modules. The microtomes are routinely used for sectioning fixed, cryoprotected tissue into slices of 30-50 µm thickness.
We provide one Leica VT1000S vibratome for sectioning fresh or fixed tissue into slices of 50-400 µm thickness.
Histological staining procedures
The facilities include ample space and resources for performing routine histological procedures such as Nissl stain for anatomical delineation and immunohistochemistry for visualization of proteins of interest. We catalogue a wide variety of antibodies as well as multiple fluorescent or chromogenic end products. We have extensive experience in troubleshooting and optimizing immunohistochemical protocols.