Single-molecule localization microscopy and single-particle tracking (SMLM and SPT)

The Nikon Eclipse Ti2 is a versatile widefield fluorescence microscope optimized for intracellular localization and live-cell tracking of proteins or RNA and detection of cell surface diffusion and membrane trafficking events at the single-particle level. The equipment is suitable for single-molecule localization using photoactivation localization microscopy (PALM), point accumulation in nanoscale topography (PAINT), highly Inclined and laminated optical sheet (HILO) microscopy, total internal reflection fluorescence (TIRF) microscopy, and quantum dot imaging, in addition to common widefield/brightfield microscopy applications. The instrument is suitable for imaging in primary cell cultures, stem cells, cell lines, or similar in vitro preparations.  

For technical specifications see the website of the Molecular Imaging Centre at the University of Bergen.