The equipment delivers high-resolution microscopic brightfield or fluorescence images of histological sections on standard (or large format) slides, with options for acquisitions of entire sections with extended focus range (projection of multiple focal planes) or multiple focal planes.
The AxioScan Z1 (Carl Zeiss) allows brightfield or fluorescence digitization of histological sections on standard or large format slides at a resolution of 0.11 micrometers per pixel in ZEN (.czi) format (for ZEN lite Blue from Carl Zeiss) with options for export to TIFF format. The instrument offers extended focus as well as acquisition of multiple focal planes.
Currently the following fluorescence filter sets are installed in AxioScan Z1, detailed description of filter set can be found via links to Zeiss’ website:
- Filter Set 38 HE (for example FITC/ GFP)
- Filter Set 43 HE (for example Cy3/ Rhodamine)
- Filter Set 49 (for example DAPI)
- Filter Set 50 (for example Cy5)
- Filter Set 64 HE
Software ‘Zen lite’ from Zeiss can be used for image viewing of ZEN format. It is a free software and please contact us for access.
Mirax Scan (Carl Zeiss) allows brightfield digitization of histological sections on standard slides (with polished edges) at a resolution of 0.22 micrometers / pixel in MIRAX format (for Pannoramic Viewer fra 3DHistech) with option for export to TIFF format. The instrument offers extended focus as well as acquisition of multiple focal planes.
Photo: Gunnar F. Lothe, Institutt for medisinske basalfag, UiO.